CytoSpark® OMNIdrop
OMNIdrop integrates droplet generation, micro-imaging/analysis, and sorting in a single instrument. It supports high-throughput functional screening, targeted single-cell sequencing workflows, and droplet-based digital assays, covering applications from antibody discovery and enzyme evolution to single-molecule quantification.
Features
- Integrated workflow: generation → analysis → sorting with compatible chips, reagent kits, and software.
- Broad assay compatibility: magnetic-bead and reporter-cell assays, nucleic-acid/protein fluorescence probes, FRET, and more.
- High-throughput single-cell sequencing: capture up to ~10⁵ cells per run for deeper maps and rare-subset discovery.
- Precise, clean sorting: disposable closed sorting chip minimizes maintenance/contamination; logic-gated, co-localized fluorescence sorting with ≥95% accuracy.
- Instrumentation options: temperature control, 384-well printing, UV sterilization, absorbance detection available as accessories.
Specifications
- Laser excitation: standard 488 nm; optional 633/405/520 nm
- Detection channels: dual fluorescence (520/30, 587/37) standard; other filters optional (e.g., 450/50, 620/30, 660/30)
- Fluorescence sensitivity: ≤10 nM FITC or ≤10 nM PE
- Droplet generation: 3 pressure control channels; speed 100–5,000 s⁻¹; millions of droplets in ~10 minutes; chip sizes for ~30/50/90 µm droplets; compatible with 1.5/5/15 mL tubes
- Reading speed: 500–1,500 s⁻¹; sorting speed: 200–1,000 s⁻¹; positives handled: 1–10 million droplets per run
- Sorting modes: bulk collection; optional 96-well single-cell printing and 8-tube strip collection; sorting accuracy ≥95%
- Instrument size: 82 × 56 × 50 cm (OMNIdrop unit)
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