CytoSpark® CSP
CytoSpark® CSP is a droplet microfluidics platform that encapsulates single cells into picoliter droplets, detects their functional readouts by fluorescence, and sorts positives at scale. It enables million-cell screens in a day with plate printing or bulk collection, accelerating single-B antibody discovery and other function-based selections.
Features
- Ultra-high throughput: process millions of cells per run with rapid detection and sorting.
- Accurate, logic-gated sorting: fluorescence co-localization and flow-cytometry-like gating improve hit quality; dielectrophoretic sorting preserves viability.
- Flexible recovery: dispense to 96/384-well plates or bulk-collect droplets to fit different downstream workflows.
- Assay versatility: supports soluble and transmembrane targets, cross-species screening, neutralizing/blocking assays, bispecifics, and TCR-T screening.
Quality control: imaging of positive droplets for monoclonality/quality inspection.
Specifications
- Lasers: 405/488/561/635 nm (customizable)
- Detection channels (PMT): DL405 / AF488 / PE / AF647 / mCherry / Cy5 (customizable)
- Droplet diameter: 30–90 µm (customizable)
- Droplet generation speed: 2,000–5,000 s⁻¹
- Droplet sorting speed: 200–1,000 s⁻¹; throughput 10¹–10⁷ per run
- Printing speed: 15–18 min per 96-well plate; modes: bulk, 96/384-well printing
- Sorting purity: >95%
- System size: Sorter 82 × 56 × 50 cm (Encapsulator listed separately in brochure overview)
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