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Product

CytoSpark® CSP

DPBio

CytoSpark® CSP is a droplet microfluidics platform that encapsulates single cells into picoliter droplets, detects their functional readouts by fluorescence, and sorts positives at scale. It enables million-cell screens in a day with plate printing or bulk collection, accelerating single-B antibody discovery and other function-based selections.

Features

  • Ultra-high throughput: process millions of cells per run with rapid detection and sorting.
  • Accurate, logic-gated sorting: fluorescence co-localization and flow-cytometry-like gating improve hit quality; dielectrophoretic sorting preserves viability.
  • Flexible recovery: dispense to 96/384-well plates or bulk-collect droplets to fit different downstream workflows.
  • Assay versatility: supports soluble and transmembrane targets, cross-species screening, neutralizing/blocking assays, bispecifics, and TCR-T screening.
  • Quality control: imaging of positive droplets for monoclonality/quality inspection.

     

Specifications

  • Lasers: 405/488/561/635 nm (customizable)
  • Detection channels (PMT): DL405 / AF488 / PE / AF647 / mCherry / Cy5 (customizable)
  • Droplet diameter: 30–90 µm (customizable)
  • Droplet generation speed: 2,000–5,000 s⁻¹
  • Droplet sorting speed: 200–1,000 s⁻¹; throughput 10¹–10⁷ per run
  • Printing speed: 15–18 min per 96-well plate; modes: bulk, 96/384-well printing
  • Sorting purity: >95%
  • System size: Sorter 82 × 56 × 50 cm (Encapsulator listed separately in brochure overview)

Technology

Antibody Discovery and Binding Kinetics

Antibody discovery and membrane kinetics research combine advanced cellular and molecular analysis methods to identify, characterize, and optimize antibodies with therapeutic or diagnostic potential. Modern workflows integrate single-cell screening, droplet...
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